Resveratrol supports and alpha-naphthoflavone disrupts growth of human ovarian follicles in an in vitro tissue culture model
نویسندگان
چکیده
منابع مشابه
In vitro culture of ovarian follicles.
This review offers a practically oriented introduction to follicle culture in vitro, focusing on mouse follicles, but with reference to other species. The main principles of follicle growth are addressed, including the constraints of tissue culture, methods of follicle isolation, and techniques for individual and collective culture of intact follicles. Culture systems that support a spherical o...
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Background: In vitro culture (IVC) of isolated preantral follicles from cryopreserved ovarian tissue might be an efficient method for enhancing mature oocytes in patients who are exposed to infertility. Materials and Methods: Ovaries of 13-day old NMRI mice were removed and randomly placed into control,needle immersed (NIV) and solid surface vitrification (SSV) groups. For vitrification, ovarie...
متن کاملP-107: Vitrification and In Vitro Culture of Mouse Preantral Follicles in Presence of Growth Factors
Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
P-23: The Effect of LIF on In Vitro Growth and Apoptosis Incidence in Vitrified Human Ovarian Tissue after 14 days culture
Background The high effectiveness of vitrification, as a cryopreservation protocol, for human oocytes and embryos is shown, whereas data on human ovarian tissue are limited. This study aimed to assessment of follicular growth, ultrastructure, and apoptosis incidence in human ovarian tissue following vitrification/warming and after culture in the presence of LIF. MaterialsAndMethods Ovarian cort...
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ژورنال
عنوان ژورنال: Toxicology and Applied Pharmacology
سال: 2018
ISSN: 0041-008X
DOI: 10.1016/j.taap.2017.11.009